منابع مشابه
Extending the viability of acute brain slices
The lifespan of an acute brain slice is approximately 6-12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously...
متن کاملPreparation of Brain Slices
Frontiers in Neural Circuits www.frontiersin.org May 2010 | Volume 4 | Article 12 | 1 clature for morphological, physiological and molecular features was agreed upon by the Petilla Interneuron Nomenclature Group (Ascoli et al., 2008). Among GABAergic interneurons, those expressing the neuropeptide somatostatin (SOM) are particularly heterogeneous in their molecular, morphological and electrophy...
متن کاملTwo-photon imaging in living brain slices.
Two-photon excitation laser scanning microscopy (TPLSM) has become the tool of choice for high-resolution fluorescence imaging in intact neural tissues. Compared with other optical techniques, TPLSM allows high-resolution imaging and efficient detection of fluorescence signal with minimal photobleaching and phototoxicity. The advantages of TPLSM are especially pronounced in highly scattering en...
متن کامل5-HT4R activation in hippocampal brain slices
5-HT4 receptor (5-HT4R) activation induces pro-cognitive effects. This might be related to stimulation of hippocampal acetylcholine (ACh) release, which has been shown for 5-HT4R agonists in in vivo models. We investigated the influence of the 5-HT4R agonists prucalopride and BIMU-8 on ACh release in rat hippocampal brain slices. In contrast to the report by Siniscalchi et al. [1], no facilitat...
متن کاملTryptophan hydroxylase activity in brain slices.
Numerous experimental data have shown that the rate of 5-hydroxytryptamine (5-HT) synthesis is enhanced by neuronal activity in mammalian central 5-HT neurons (Hamon and Glowinski, 1974; Knapp et al., 1975; Neckers et al., 1977; Boadle-Biber et al., 1983). The change of 5-HT synthesis may be due to rapid and reversible modifications of the activity of tryptophan hydroxylase (EC 1.14.16.4), whic...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2018
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2699